VA Research Core

SkyScan 1276 microCT

• Integrated physiological monitoring
• On screen dose meter
• Highest nominal resolution currently at 2.8microns

Vevo 2100 Imager

• 30-micron resolution up to 740fps
• Color and doppler
• Blood flow quantification
• Volumetric analysis

Intravital Nikon Ti2

• 100 fps near real time imaging
• 25mm field of view
• A1r-MP Multiphoton confocal system

IVIS Spectrum

• High throughput with 23cm field of view
• 20-micron resolution
• 2D and 3D optical tomography

Investigators must be performing research at VA facilities to utilize imaging equipment

Other services: CODA blood pressure monitor, treadmill, and smoking machine.

NextSeq 550

• High throughput sequencer
• Targeted or full genome sequencer
• Up to 16 transcriptomes
• 25-120 Gb output
• 130-400 million reads per run
• 2x150 bp in length
• 1 human whole genome
• 12 exomes
• Kits: www.illumina.com

Freedom EVO (Tecan) 100

• Liquid Handler
• 30 grids of worktable space
• Assists with library preps
• Nucleic acid purification
• PCR setups
• Protein purifications

ddSEQ

• Single Cell isolator
• Coencapsulates single cells and barcodes into subnanoliter droplets

TapeStation

• Automated electrophoresis solution for the sample quality control of DAN and RNA samples
• It is suitable to analyze size, quantity, and integrity of your samples

Nanostring nCounter Sprint

• RNA, DNA and protein profiling
• 10s up to 800 targets
• No cDNA or library preps needed
• RNA 1-50ng needed
• DNA 5ng-300ng
• Protein 250ng-2.5ug
• Panel options: NanoString Technologies PanelPro

Leica SP8 Confocal with White Light Laser

• White light laser capabilities:
  • Excites up to eight tunable laser lines simultaneously
  • 470-670nm excitation range with 1nm resolution
• Time gated detectors and motorized prism detector system:
  • Allows for Lambda scans
  • High microscope setting variation allows for best quality images per samples
• Near real-time deconvolution per pixel

Zeiss Axioplan

• O2 and CO2 stage incubator capable of doing hypoxia experiments
• Zeiss deconvolution software available
• DIC hardware

Zeiss Discovery V20

• Fluorescent and video capabilities stereo scope
• 5xand 1x objectives

Keyence

• 4 color fluorescence
• High throughput
• Multiple well plate imaging
• User friendly

BD LSR 2

• Four lasers allow for up to 18 colors
• Capable of up to 20,000 events per second
• Four lasers: - 355, 405, 488, and 642

BD Symphony A5SE

• Five lasers and 48 detectors
• Capable of up to 40,000 events per second
• 48 fluorescent detectors that cover the full spectrum of visible light off standard laser lines: 
  • UV (349 nm): 10 detectors between 365 nm–850 nm 
  • Violet (405 nm): 14 detectors between 415 nm–880 nm 
  • Blue (488 nm): 9 detectors between 500 nm–849.5 nm 
  • Yellow-Green (561 nm): 9 detectors between 570 nm–850 nm 
  • Red (637 nm): 6 detectors between 645 nm–810 nm

Bioplex200

• Suspension array system Multiplexing up to 100 Biomolecules on a single sample
• Magnetic plate washer for pre-run preparation
• Panel Options: Bio-Plex Multiplex Immunoassays

CytoFlex LX

• 96 well plate loader
  • Customizable wash and mix cycles
  • Multiple experiments on same plate
• Five lasers - 375(NUV), 405, 488, 562, and 642

ImageStreamX Mk.2

• Flow analysis accompanied by single cell images
  • Protein localization and co-localization
  • Phagocytosis
  • Cell to cell interaction
• Up to 10 fluorescent markers at once
• Four lasers - 405, 488, 562, and 642

• Ultraforce DXA X-ray
• Micro CT Scan
• Vevo Imaging
• Intravital Scope
• IVIS Imaging

Prices for Ultraforce DXA X-ray, Micro CT Scan, Vevo Imaging, Intravital Scope, and IVIS Imaging include room rate plus instrument rate. Contact us for more information.

VA-funded hourly prices

• Zeiss axioplan - $25 
• Leica SP8 - $35
• Symphony A5SE- $90
• LSR2 BD - $50
• Cytoflex LX - $40
• ImagestreamX - $125
• NextSeq550 - $400
• Nanostring sprint - $250
• Bioplex200 - $200
• Keyence Scanning scope - $10
• TapeStation - $10

Other Services

• Training rate - $50
• Analysis rate - $50

Non-VA funded hourly prices

• Zeiss axioplan - $35 
• Leica SP8 - $50
• Symphony A5SE-$115
• LSR2 BD - $70
• Cytoflex LX - $60
• ImagestreamX - $150
• NextSeq550 - $500
• Nanostring sprint - $300
• Bioplex200 - $250
• Keyence Scanning scope - $20
• TapeStation - $15

Other Services

• Training rate - $75
• Analysis rate - $75